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Conventional and nested PCR using different primers than those of the BioMarkTM system were used to confirm the presence of the detected pathogens in the samples (Table 1). Amplicons were sequenced by Eurofins MWG Operon (Ebersberg, Germany) and assembled using the BioEdit software (Ibis Biosciences, Carlsbad, USA). An online BLAST (National Center for Biotechnology Information) was used to identify the sequenced organisms. 153554b96e
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